Use of Adapted Diffusion Method as Preliminary Assay for Antifungal Biocides Efficacy Currently Used in Decontamination

http://www.revistadechimie.ro 1978 In the last decades, significant progress has been made in the mechanisms of antibacterial action of biocides understanding. However, information on biocides action against fungi remained yet sparse. Studies of the biocides mode of action on fungi and yeasts, suggested that, unlike antibiotics, which act selectively on cellular targets; they act on one or more than one location, like: cell wall, plasma membrane, thiol groups of the proteins and enzymes, ribosomes and DNA [1-4]. An important issue, requiring multifaceted defence measures, is the presence of fungal or biofilm-related infections, more frequently signalled, and not easy to eradicate, became a significant threat, confirmed by the fungal resistance mechanisms [5]. In parallel with the rent animals intensive industrial systems expansion, an alarming increase in resistance occurrence and distribution of various microorganism species to one or more than one medicinal substances, antibiotics / antifungals, biocides / decontaminating products has been noticed, all followed by negative implications in the control of diseases both, in humans and animals [6-8]. Therefore, in order to survive to the biocides exposure, fungi act via activation of a number of resistance mechanisms to reduce the toxic concentrations of the chemical compounds [9]. Important steps, from non-standardized to standardized procedures, have been proposed in the last decade, into the specialists aid, antifungal susceptibility testing (AST) becoming an accepted methodology for human and veterinary mycology [10, 11]. The Clinical and Laboratory Standards Institute (CLSI), and the domain researchers published new methods for antifungal susceptibility testing. In this respect, the Diffusion Qualitative Method (DQM) for determining the susceptibility of fungi to biocides is based on principles used in antibiograms and antifungigrames and can be Use of Adapted Diffusion Method as Preliminary Assay for Antifungal Biocides Efficacy Currently Used in Decontamination